PHYSICOCHEMICAL CHARACTERIZATION OF PITUITARY FOLLICLE-STIMULATING HORMONE
نویسندگان
چکیده
منابع مشابه
Physicochemical Characterization of Pituitary Follicle-stimulating Hormone
The physiochemical characteristics of the follicle-stimulating hormone (FSH) from whole sheep pituitary glands have been studied. The hormone behaves as a single protein in electrophoresis, diffusion, and ultracentrifugation. It has an isoelectric point at pH 4.5 and a molecular weight of 67,000 and contains 1.23 per cent hexose and 1.51 per cent hexosamine. The amino acid composition has also ...
متن کامل[Purification of follicle-stimulating hormone from human pituitary glands].
A highly purified follicle-stimulating hormone from human pituitary glands has been prepared by ammonium sulfate and ethanol fractionation, gel filtration on Sephadex G-100, ion exchange chromatography on carboxymethyl cellulose, zone electrophoresis on cellulose columns, and preparative continuous flow polyacrylamide gel electrophoresis. The final product has shown lO,OOO-fold purification ove...
متن کاملPurification of Follicle-stimulating Hormone from Human Pituitary Glands*
A highly purified follicle-stimulating hormone from human pituitary glands has been prepared by ammonium sulfate and ethanol fractionation, gel filtration on Sephadex G-100, ion exchange chromatography on carboxymethyl cellulose, zone electrophoresis on cellulose columns, and preparative continuous flow polyacrylamide gel electrophoresis. The final product has shown lO,OOO-fold purification ove...
متن کاملData on the characterization of follicle-stimulating hormone monoclonal antibodies and localization in Japanese eel pituitary
Monoclonal antibodies were generated against recombinant follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica; rec-FSH was produced in Escherichia coli and purified using Ni-NTA Sepharose column chromatography. In support of our recent publication, "Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japan...
متن کاملIsolation and characterization of subunits from equine pituitary follicle-stimulating hormone.
Equine follicle-stimulating hormone (FSH) was dissociated into two noncovalently linked subunits, (Y and /3. This was accomplished by incubation of FSH in 8 M urea at 37” for 20 hours followed by ion exchange chromatography on DEAESephadex A-50. This resulted in complete separation of the subunits. The individual subunits were purified by gel filtration on Sephadex G-100 columns. The results of...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1952
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.35.4.629